STAT3 but Not STAT5 Contributes to the Protective Effect of Electroacupuncture Against Myocardial Ischemia/Reperfusion Injury in Mice

Electroacupuncture (EA) can help reduce infarct size and injury resulting from myocardial ischemia/reperfusion (I/R); however, the underlying molecular mechanism remains unknown. We previously reported that STAT5 plays a critical role in cardioprotective effect of remote ischemic preconditioning (RIPC). Here, we assessed effects electroacupuncture pretreatment (EAP) on I/R presence and/or absence Stat5 mice investigated whether EAP exerts its STAT5-dependent manner. Adult fl / Stat5- cKO were exposed to at Neiguan (PC6) for 7 days before induction by left anterior descending (LAD) coronary artery ligation. The (IS), area risk, apoptotic rate cardiomyocytes detected. RT-qPCR western blotting used measure gene protein expression, respectively, homogenized heart tissues. RNA-seq was identify candidate genes pathways. Our results showed decreased IS cardiomyocyte apoptosis. further found activated but not mice, whereas opposite observed STAT3. Following EAP, levels antiapoptotic proteins Bcl-xL, Bcl-2, p-AKT increased , while interleukin 10 (IL-10) both cKO. expression profile tissues different between with EAP. Importantly, top 30 DEGs under enriched IL-6/STAT3 signaling pathway. revealed first time protective following attributable to, dependent on, STAT5. Additionally, could activate STAT3 gene, also antiapoptotic, survival, anti-inflammatory